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International Journal ofMolecular SciencesArticleInterferon Induces the Apoptosis of Cervical Cancer HeLa Cells by Activating both the Intrinsic Mitochondrial Pathway and Endoplasmic Reticulum Stress-Induced PathwayWei-Ye Shi 1 , Cheng Cao two and Li Liu 1, *1*Department of Microbiology, Institute of Fundamental Medical Sciences, Chinese Academy of Health-related Sciences Peking Union Medical College, Beijing 100005, China; [email protected] Beijing Institute of Biotechnology, Academy of Military Medical Sciences, 27 Taiping Rd., Haidian District, Beijing 100850, China; [email protected] Correspondence: [email protected]; Tel.: +86-10-6915-Academic Editor: Anthony LemariReceived: 29 July 2016; Accepted: 27 October 2016; Published: two NovemberAbstract: The interferon (IFN-) has been typically utilized as a sensitizing agent for the treatment of various malignancies including hepatocellular carcinoma, malignant melanoma, and renal cell cancer by advertising the apoptosis of thesetumor cell sorts.Price of N-Desethyl amodiaquine dihydrochloride On the other hand, the impact of IFN- on cervical cancer remains unknown.PMID:22664133 In this study, HeLa cells were utilised as a testing model for the treatment of IFN- on cervical cancer. The outcomes indicate that IFN- markedly inhibits the proliferation and induces the apoptosis of HeLa cells. The activation of caspase 3, the up-regulation of each Bim and cleaved poly (ADP-ribose) polymerase (PARP) 1, the down-regulation of Bcl-xL, also as the release of cytochrome c from mitochondria had been considerably induced upon IFN- therapy, indicating that the intrinsic apoptotic pathway could be activated by IFN- remedy. Moreover, caspase 4–which is involved inside the endoplasmic reticulum (ER) stress-induced apoptosis–was activated in response to IFN- treatment. Knocking down caspase 4 by smaller interfering RNA (siRNA) markedly lowered the IFN–mediated cell apoptosis. On the other hand, no substantial modifications in the expressions of caspases eight and 10 have been observed upon IFN- treatment, indicating that the apoptosis caused by IFN- may be independent of the extrinsic apoptotic pathway. These findings suggest that IFN- may possess a.
