Product Name :
Nanog Rabbit Polyclonal Antibody
Predicted band size :
Observed band size :
Synonyms:
Embryonic stem cell specific homeobox protein (Nanog) antibody ENK antibody FLJ12581 antibody hNanog antibody Homeobox protein NANOG antibody Homeobox transcription factor Nanog antibody homeobox transcription factor Nanog-delta 48 antibody NANOG antibody Nanog homeobox antibody NANOG_HUMAN antibody
Function :
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5′-TAAT[GT][GT]-3′ or 5′-[CG][GA][CG]C[GC]ATTAN[GC]-3′. When overexpressed, promotes cells to enter into S phase and proliferation.
Antibody Type:
Rabbit Polyclonal Antibody
Immunogen :
KLH conjugated synthetic peptide derived from mouse Nanog 101-200/305
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, ELISA, IHC-P, IF, ICC
Coupling :
unconjugated
Clone number:
RRID:
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at -20℃ for one year.91511-38-5 web Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃.Ethyl 5-bromo-6-chloropicolinate manufacturer When reconstituted in sterile pH 7.PMID:33555643 4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
Storage buffer solution:
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
34 KDa
Positive control :
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.